nucleic acids host:plantpath.unl.edu

Listing 1 - 8 from 8 for nucleic acids

UNL Department of Plant Pathology Faculty
... , Research Assistant Professor James Steadman, Professor-Disease Management/Resistance Roy French, USDA/ARS - Viruses and Nucleic Acids Drake Stenger, USDA/ARS - Wheat Virology Deanna Funnel, USDA Sorghum Pathology James Van Etten, Professor ...
plantpath.unl.edu

Les Lane's home page
... (3rd world friendly) Stabilizing and "resurrecting" dithiothreitol stock solutions - 3rd world friendly. Precipitating proteins and nucleic acids - why waste time dialyzing? (3rd world friendly) Brome mosaic virus - introduction and properties, Chenopodium quinoa ...
plantpath.unl.edu

Gel electrophoresis tips and troubleshooting
... Probes makes many useful dyes including Sypro ruby for proteins and Sybr Gold for staining nucleic acids in gels. Gel problems No SDS in wells - protein precipitates as it enters running gel ...
plantpath.unl.edu

Biological Detergents - the basics
... guide 6- Polyionic detergent micelles can precipitate oppositely charged polyions (e.g. CTAB precipitation of nucleic acids) 7- Detergent head groups dictate surface interactions. Headgroups of cationic detergents bind to anionic glass ...
plantpath.unl.edu

Phenol extraction
... phenol. PNAS 12, 433- 438, 1926) and lipids leaving water soluble matter (carbohydrates, nucleic acids, etc.) in the aqueous layer. Particulate and "ambiguous" matter remain insoluble. Phenol ... from phenol extracted nucleic acids CTAB (cetyltrimethylammonium bromide, Bellamy, A.R., and Ralph, R.K. Meth Enzymology 12B, 156; 1968) is commonly used to precipitate nucleic acids. It leaves neutral ...
plantpath.unl.edu

Ammonium acetate as a buffer
... acetate as a buffer Home Buffer page Spreadsheet titration Precipitating proteins and nucleic acids Ammonium acetate Ammonium acetate as a buffer You nitwit! Ammonium acetate is a salt, not ... and nucleic acid purifications It's obviously easy to prepare, but are there other advantages? A big advantage is solubility in acetone and methanol (like LiCl). You can precipitate proteins or nucleic acids ...
plantpath.unl.edu

Stabilizing and "resurrecting" DTT (dithiothreitol) stock solutions
... Stabilizing and "resurrecting" DTT (dithiothreitol) stock solutions Home Gel electrophoresis tips Precipitating proteins and nucleic acids Reduction alkylation protocol Dithiothreitol (DTT) solutions Structure of dithiothreitol, DTT History of DTT Stabilizing and " ...
plantpath.unl.edu

Precipitating macromolecules with organic solvents
... solvents General principles Acetone and alcohols (EtOH, MeOH) precipitate proteins and nucleic acids. Avoid inorganic salts which coprecipitate. Precipitate small amounts so that precipitate ... a common deglycosylation reagent. Although proteins won't precipitate directly from acids, neutralizing organic acids with an organic base (I use N-ethylmorpholine) permits straightforward precipitation ...
plantpath.unl.edu